7 research outputs found
Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
Efficient treatments in bacterial infections require the fast
and accurate recognition of pathogens, with concentrations as
low as one per milliliter in the case of septicemia. Detecting
and quantifying bacteria in such low concentrations is
challenging and typically demands cultures of large samples of
blood (~1 milliliter) extending over 24-72 hours. This delay
seriously compromises the health of patients. Here we
demonstrate a fast microorganism optical detection system for
the exhaustive identification and quantification of pathogens in
volumes of biofluids with clinical relevance (~1 milliliter) in
minutes. We drive each type of bacteria to accumulate antibody
functionalized SERS-labelled silver nanoparticles. Particle
aggregation on the bacteria membranes renders dense arrays of
inter-particle gaps in which the Raman signal is exponentially
amplified by several orders of magnitude relative to the
dispersed particles. This enables a multiplex identification of
the microorganisms through the molecule-specific spectral
fingerprints
Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
Efficient treatments in bacterial infections require the fast
and accurate recognition of pathogens, with concentrations as
low as one per milliliter in the case of septicemia. Detecting
and quantifying bacteria in such low concentrations is
challenging and typically demands cultures of large samples of
blood (~1 milliliter) extending over 24-72 hours. This delay
seriously compromises the health of patients. Here we
demonstrate a fast microorganism optical detection system for
the exhaustive identification and quantification of pathogens in
volumes of biofluids with clinical relevance (~1 milliliter) in
minutes. We drive each type of bacteria to accumulate antibody
functionalized SERS-labelled silver nanoparticles. Particle
aggregation on the bacteria membranes renders dense arrays of
inter-particle gaps in which the Raman signal is exponentially
amplified by several orders of magnitude relative to the
dispersed particles. This enables a multiplex identification of
the microorganisms through the molecule-specific spectral
fingerprints
Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
Efficient treatments in bacterial infections require the fast
and accurate recognition of pathogens, with concentrations as
low as one per milliliter in the case of septicemia. Detecting
and quantifying bacteria in such low concentrations is
challenging and typically demands cultures of large samples of
blood (~1 milliliter) extending over 24-72 hours. This delay
seriously compromises the health of patients. Here we
demonstrate a fast microorganism optical detection system for
the exhaustive identification and quantification of pathogens in
volumes of biofluids with clinical relevance (~1 milliliter) in
minutes. We drive each type of bacteria to accumulate antibody
functionalized SERS-labelled silver nanoparticles. Particle
aggregation on the bacteria membranes renders dense arrays of
inter-particle gaps in which the Raman signal is exponentially
amplified by several orders of magnitude relative to the
dispersed particles. This enables a multiplex identification of
the microorganisms through the molecule-specific spectral
fingerprints